Sooner Scientific Catalog

P.O. Box 180 - Garvin, OK 74736 - 405-237-0302

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Description - The Sooner Scientific Electro-Eluter/Concentrator * is designed to elute and concentrate macromolecules simultaneously from acrylamide or agarose gels. Typical applications include recovery of proteins from SDS-acrylamide gels (1,2) and of restriction enzyme-treated DNA from agarose gels.

Each unit comes complete with:

Blocks may be ordered with two loading capacities: 2.0ml (Cat.# DBA-102) and 5.0ml (Cat.# DBA-105). The DBT-040 will accommodate up to seven 2.0ml blocks side by side or five 5.0ml blocks.* Licensed under U.S. Patents 4,159,933 and 4,164,464

REFERENCES:
(1) Hunkapillar, M.W.; Lujan, E.; Ostander, F.; and Hood, L.E.--- "Isolation of Microgram Quantities of Proteins from Polyacrylamide Gels for Amino Acid Sequence Analysis," Methods of Enzymology. Vol 91, 1983, pages 227-236.
(2) Watson, J.; Barton-Frank, M.; Mochizuki, D.; and Gillis, S.; "The Biochemistry and Biology of Interleuken 2," Lymphokines, Vol.6, Academic Press, 1982, pages 95-116.

Eluter/Concentrator Blocks - Each block consists of a precision-machined sample migration channel and two silicone-Teflon washer/open-top cap assemblies for holding dialysis membranes securely in place. Smaller block has a loading chamber capacity of 2.0ml, a total migration channel volume of 5.0ml, and a sample recovery volume of 200µl. Larger block has a loading chamber capacity of 5.0ml, a total migration channel volume of 12.0ml, and a sample recovery volume of 500µl.

Elution Procedure - Blocks are prepared for electrophoresis by securing non-charged membranes into place with washers and open-top cap assemblies (as shown in figure 1). The gel slice is top-loaded onto the anode membrane. The entire block and electrode chambers are then filled with running buffer. Blocks are placed in unit for electrophoresis.

The mirrored bottom of the chamber allows easy detection of any bubbles that may collect beneath each black cap. All bubbles should be flushed from the membrane with a syringe and bent needle to prevent any interference of current (ionic) flow through the block. During electrophoresis, sample migrates out of gel, across buffer bridge, and concentrates above cathode membrane in sample recovery channel. Upon completion of electrophoresis most of the running buffer is pipetted off and discarded. Sample may then be recovered by resuspending in a small amount of remaining buffer.

Buffer Cycling System - The Sooner Scientific three-chamber Electro-Eluter is designed to allow a buffer cycling system as described by Hunkapillar et al (1983). The buffer drain outlets on the side of the two electrode chambers allow overflow buffer to drop into the mixing chamber. The mixed buffer is then pumped back into the electrode chambers. This prevents the buildup of a pH gradient which can occur during long-term electrophoresis, interfering with the ionic migration of the protein or DNA sample. This buffer cycling system also eliminates the need to change buffer during electrophoresis.

Special Features -

 

Ordering/Price Information - Please see page 30 or the index.


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