Sooner Scientific Catalog

P.O. Box 180 - Garvin, OK 74736 - 405-237-0302

| Catalog Index | Price Index | E-mail Us |

• Accessories for Sequencing Systems
  • Combs
  • Spacer Sets
  • Glass Plates and Aluminum Heat Dispersion Plates
  • White Clamps
  • Gel Sealing Tape
  • Quick-Disconnect Coupling
  • Bar Clamp
  • Ordering/Price Information

Rectangular Tooth Combs: Rectangular tooth combs are precision-machined polycarbonate and are available in a wide variety of sizes for both 20cm and 33cm wide units. Sequencing combs for the 20cm wide unit are available in three thicknesses: 0.2mm, 0.4mm, and 0.5mm; and are offered with 8, 12, 14, 16, 17, 20, 28, 32, or 35 teeth. Teflon¨* combs for protein or preparative gels can also be ordered for the 20cm unit with thicknesses of 0.75mm, 1.0mm, 1.5mm, 2.0mm, or 3.0mm with 1, 2, 3, 4, 8,12, 14, 16, 17, 20, 28, 32, or 35 teeth. Sequencing combs for the 33cm wide sequencer are available in three thicknesses: 0.2mm, 0.4mm, or 0.5mm, and are offered with 8, 16, 24, 31, 34, 50, 60, or 63 teeth. Teflon¨ combs for preparative or protein gels for the 33cm wide unit can be ordered with thicknesses of 0.75mm, 1.0mm, 1.5mm, 2.0mm, or 3.0mm with 3, 5, 6, 8, 12, 16, 24, 31, 34, 50, 60, or 63 teeth.

Sooner Scientific offers the researcher the option to have numbers printed above the wells of the sharkstooth combs. This convenient feature allows the researcher to keep track of which sample goes into each application site during loading. Shown above with microtrough glass plates. These specially designed plates enable quick and convenient loading of small amounts of sequencing sample.

Combs for Multichannel Pipettor Loading: Sooner Scientific has designed rectangular tooth and sharkstooth combs with microtitre plate spacing for multi-channel pipettor loading. Rectangular combs with this feature are the 17 and 35-well combs for the 20cm wide units and the 31 and 63-well combs for the 33cm wide units. The Sharkstooth combs that can be loaded with a multichannel pipettor are the 2.25mm, 3.0mm, and 4.5mm point to point combs. The 2.25mm point to point combs correspond to our 126-well comb for the 33 cm wide sequencers or our 70-well comb for the 20cm wide sequencer, and a multichannel pipettor would load every 4th well. The 3.0mm point to point combs correspond to our 94-well comb for the 33 cm wide sequencers or our 52-well comb for the 20cm wide sequencer, and a multichannel pipettor would load every 3rd well. The 4.5mm point to point combs correspond to our 62-well comb for the 33 cm wide sequencers or our 34-well comb for the 20cm wide sequencer, and a multichannel pipettor would load every other well.

Spacer Sets - Linear Wedge Spacers: Linear wedge spacers are offered in 3 different ratios, 1:2, 1:3, and 1:4. They are available to fit 42cm, 52cm, and 62cm glass plate lengths for Sooner Scientific's sequencing equipment and can be special ordered to fit other suppliers' equipment as well. These linear wedge spacers are precision machined from a single piece of grey PVC. A bottom spacer for the wedge sets is available as an option which some researchers have found useful for preventing leakage. The thinner top part of the spacers can start at either 0.2mm (.010") or 0.4mm (.015") for all three different ratios of wedge spacers.

Flat Spacer Sets: Spacer sets for both the 20cm wide and the 33cm wide sequencers are available in three thicknesses (0.2mm, 0.4mm, and 0.5mm) and for gel lengths of 22cm, 32cm, 42cm, 52cm, 62cm, 82cm, and 102cm. Spacer sets for preparative or protein gels can be ordered with thick-nesses of 0.75mm, 1.0mm, 1.5mm, 2.0mm, and 3.0mm.

Exponential Wedge Spacers: Exponential wedge spacers differ from linear in that two sets of spacers are used for each gel. The first is a flat spacer set, followed by a wedged pair. The spacers are assembled end to end on the glass plate and the gel is poured using standard techniques. This system maximizes the "wedge spacer effect", allowing routine sequence penetration of 400-800 bases. The exponential wedge spacer sets are available in two different ratios, 1:3 and 1:5. They are available to fit 42cm, 52cm, 62cm, 82cm, or the 102cm glass plate lengths for Sooner Scientific's sequencing equipment.

Glass Plates and Aluminum Heat Dispersion Plates - Glass Plates: Glass plates sets are 3/16" thick and can be ordered in lengths of 22cm, 32cm, 42cm, 52cm, 62cm, 82cm, or 102cm. Standard notched plate sets are provided with the kits. Unnotched plate sets with detached rubber ears can also be ordered. German tempex glass (pyrex) is available for special applications. All glass plates are edge sanded and polished for safety and durability.

Aluminum Heat Dispersion Plates: Ultra-flat machine-surfaced 1/4" thick aluminum plates are safety coated and are electrically non-conductive. They can be ordered to clamp behind the glass plate sandwich during electrophoresis. The use of these heat dispersion plates effectively eliminates the "smile effect" by evenly distributing the heat generated during electrophoresis. They also keep pre-run times to a minimum, unlike those systems which use extended upper buffer chambers. Aluminum plates are available for all glass plate sizes for the adjustable systems. (Dedicated Height and Dual Dedicated Sequencers include built-in aluminum heat dispersion plates.)

White Clamps - Sooner Scientific now has available molded polypropylene spring clamps for use with the Dedicated Height and Adjustable Nucleic Acid Sequencers. These clamps are designed specifically for this application and feature a stainless steel spring. There are two different types of clamps for sequencing applications. The clamps shown above are Cat. # FOB-0002 and are used for holding thinner sandwiches together. Cat. # FOB-0001 is for clamping the glass plates to the upper reservoir and holding the aluminum heat dispersion plates in place.

Gel Sealing Tape - Yellow mylar tape is available for the sealing of sequencing glass plate sets. Each roll of tape is 72 yards long with choices of 1" width (Cat. # FS-72-10) or 1.5" width (Cat. # FS-72-15).

Quick-Disconnect Coupling - The quick-disconnect tubing coupling (body, Cat. # PCB-B and insert, Cat. # PCB-I) is a convenient accessory for simplifying buffer disposal. This coupling features an automatic shut-off valve and can be ordered to replace the standard tubing adapters supplied with Sooner Scientific Sequencing Systems. The coupling insert with shut-off valve threads into the buffer reservoirs, while the coupling body attaches to the drainage tubing. To drain reservoir, simply snap coupling body onto coupling insert and the valve will open. When the coupling is disconnected the valve automatically closes.

Bar Clamp - Sooner Scientific features bar clamps for use with the Adjustable Sequencing System. (Cat. # FOB-1000 for the 20cm wide units, and Cat. # FOB-2000 for the 33cm wide units.) Bar clamps are standard with the Dedicated Height and Dual Dedicated Height Sequencing Systems. The clear acrylic bar clamp evenly compresses the glass plate sandwich to the upper reservoir gasket, replacing bulldog spring clamps. The bar clamp is particularly useful for two reasons:

1. Combs receive equal pressure along the entire width of the glass plate sandwich, eliminating capillary leakage between samples.

2. Glass plate fractures due to the "bending" that can occur with bulldog clamps is eliminated, resulting in longer plate life.

Ordering/Price Information - Please see page 4 or the index.

<-previous page | next page->

| Home | Information | What's New | E-mail Us |