Sooner Scientific Catalog
Sooner Scientific Catalog
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STANDARD GELLING AND MELTING & POINT AGAROSES
SeaQualite' Standard Analytical Grade AGAROSES: The agaroses of SeaQualite SQA-01100, SQA-02100, SQA-03025, SQA-04025 and SQA 05100 family differ mainly in electroendosmosis (EEO) values. These values are very important in macromolecular separation, especially proteins. All SeaQualite SQA-01100, SQA-02100, SQA-03025, SQA-04025 and SQA-05100 agaroses can be mixed and matched to meet desired EEO and performance specifications.
SeaQualite SQA-01100: Agarose with very low EEO values (<0.13).
-Especially recommended for preparation of analytical gels for sharp resolutions of nucleic acid fragments with sizes greater than 1000bp.
-Suitable for blotting assays (Northern, Southern,..)
-No detectable RNase and DNase activity.
-May be used at concentrations between 0.8-2% with all typical buffer systems.
-Also recommended for radial immunodiffusion and Ouchterlony techniques.
APPROPRIATE AGAROSE CONCENTRATION FOR DNA SIZES
SeaQualite Agarose |
||
| 1X TAE BUFFER | Gel Concentration | 1X TBE BUFFER |
| Size Range (bp) | % | Size Range (bp) |
|
SQA-01100 |
||
|
20000-1000 |
0.6 |
15000-1000 |
|
12000-500 |
0.8 |
10000-500 |
|
8000-300 |
1.0 |
7000-250 |
|
6000-200 |
1.2 |
5000-200 |
|
3500-100 |
1.5 |
2000-50 |
SeaQualite SQA-02100: Agarose with greater EEO values (0.16)-0.19) than SeaQualite SQA-01100.
-Recommended for electrophoresis of serum proteins, immunoelectrophoresis.
-May also be used in nucleic acid electrophoresis.
SeaQualite SQA-03025: Agarose with greater EEO values (0.23-0.26) than SeaQualite SQA-02100.
-Suitable for electrophoresis of serum proteins, immunoelectrophoresis and counterimmunoelectrophoresis.
SeaQualite SQA-04025: Agarose with greater EEO values (>0.30) than SeaQualite SQA-03025.
-Especially recommended for counterimmunoelectrophoresis and immunoelectrophoresis, especially serum globulins (IgG, IgM). Presents high cathodal migration.
SeaQualite SQA-05100 Molecular Biology Grade:
Highly purified agarose with very low EEO values certified by strict quality control test procedures.
-Strongly recommended for DNA preparative gels in routine molecular biology techniques.
-The DNA (>1000bp) recovered from agarose gels after electrophoresis can be used in enzymatic processes (restriction, ligation,...)
-Suitable for blotting assays (Northern, Southern,...)
-No detectable DNase or RNase activity.
-Low DNA binding.
Agarose is a neutral polysaccharide with a very high gellifying power. Structurally, it is a very linear polymer of high molecular weight (above 120.000 Daltons). Formed by repetitive units (1) where -R is either -H or -CH3. Agarose SeaQualite SQA-01100, SQA-02100, SQA-03025, SQA-04025 and SQA- 05100 are the results of a technical breakthrough in algal research and processing. These innovations have made possible substantial improvements in certain product attributes such as moisture, ash and sulfate content, turbidity and especially gel strength.
USES IN BIOCHEMISTRY and MOLECULAR BIOLOGY: For analytical and preparative separation techniques such as; DIFFUSIONS and IMMUNODIFFUSION.
ELECTROPHORESIS: For protein, nucleic acids and macromolecules having an electrical charge.
IMMUNOELECTROPHORESIS: For techniques such as counterelectrophoresis and electroimmunodiffusion (Laurell Rocket technique).
AGROSE BEADS PREPARATION: For Gel Permeation and Affinity Chromatography. For these uses, the type indicated is SQA-05100, low eletroendosmosis.
CELL CULTURE: Semi-solid media preparations for cell cultivation and cloning.
CELL AND ENZYME IMMOBILIZATION: To be used as catalytic beds for enzyme reactors.
ADVANTAGES
Our experience of working with different types of seaweed all over the world has enabled us to obtain greater yields and batch to batch consistency.
Our agarose SeaQualite SQA-01100, SQA-02100, SQA-03025, SQA-04025 and SQA 05100 also offers the following advantages:
1. The presents of more free hydroxyl groups that can serve as fixation points increases the capacity of SeaQualite SQA-01100, SQA-02100, SQA-03025, SQA-04025 and SQA-05100 for derivatization to activate for affinity chromatography gels. This allows coupling of enzymes, antigens, antibodies and other substances to the gel structure. Chemical cross-linking of the gel produces a gel of exceptional mechanical properties as well as thermal stability. The cross-linked gel can be autoclaved for sterilization.
2. Extraordinary mechanical resistance for more reliable and easier handling.
3. Possibility of varying pore size in accordance with particle size by modifying the gel concentration, thus improving resolution in different electrophoretic processes.
4. Easy preparations of the gel by simple dilution in aqueous buffers either by standard boiling or by using a microwave oven.
5. Greater thermal stability derived from the very high gellifying hysteresis. (Hysteresis, the difference between gelling and melting temperatures.)
6. Enhanced fraction identification and quantification as a result of the excellent transparency of the gels, both in visible and ultraviolet regions.
7. Exceptionally low absorption of florescent stains, promoting efficient washing to eliminate excess staining reagents.
8. Absence of toxicity thus avoiding neurotoxicity caused by polyacrylamide.
VARIETIES
Agaroses SeaQualite SQA-01100, SQA-02100, SQA-03025, SQA-04025 and SQA-05100 comes in five varieties ranging from very low EEO, low EEO, medium EEO, high EEO and special high EEO for different uses. (Counterimmunoelectrophoresis, for example, requires high value EEO.) All of them are miscible, thus permitting preparation of gels with the exact desired EEO value.
For electroendosmosis determination, the technique followed is as described by Wieme, working with barbital-barbitate in a pH 8.4 buffer.
APPROPRIATE AGAROSE CONCENTRATION FOR DNA SIZES
|
SeaQualite Agarose |
||
|
1X TAE BUFFER |
Gel Concentration |
1X TBE BUFFER |
|
Size Range (bp) |
% |
Size Range (bp) |
|
SQA-05100 |
||
|
20000-1000 |
0.6 |
15000-1000 |
|
12000-500 |
0.8 |
10000-500 |
|
8000-300 |
1.0 |
7000-250 |
|
6000-200 |
1.2 |
5000-200 |
|
3500-100 |
1.5 |
2000-50 |
AGAROSE EQUIVALENCE CHART
Sooner Scientific |
BMA |
Sigma |
Bio-Rad |
Gibco |
Promega |
Fisher |
| SeaQualite SQA-01100 | SeaKem LE | A6013, A0169 | 162-0125,-0126 | 15510-019,027 | LE Anal Grade | BP-160 |
| SeaQualite SQA-05100 | SeaKem GTG | A9539 | 162-0133,-0134 | No Equivalence | No Equivalence | BP-1356 |
| SeaQualite SQA-02100 | SeaKem ME | A6877, A9918 | No Equivalence | No Equivalence | No Equivalence | BP-161 |
| SeaQualite SQA-03025 | SeaKem HE | A6138, A9793 | No Equivalence | No Equivalence | No Equivalence | BP-162 |
| SeaQualite SQA-04025 | SeaKem HHE | A3643, A9668 | 162-0001 | No Equivalence | No Equivalence | No Equivalence |
| SeaQualite SQA-06025 | SeaKem HGT | A3768, A7174 | 162-0102 | No Equivalence | No Equivalence | BP-164 |
| SeaQualite SQA-07025 | No equivalence | A7299 | No Equivalence | No Equivalence | No Equivalence | No Equivalence |
| SeaQualite SQA-08025 | No equivalence | No equivalence | 162-0137,-0138 | No Equivalence | No Equivalence | No Equivalence |
BIBLIOGRAPHY
* R.J. Wieme (1965). "Agar gel electrophoresis." Elsevier, Amsterdam.
* H. Determan (196(). "Chromatographie sur gel." Masson et Cie.< Paris.
* L. Fisher (1974). "An introduction to gel chromatography". North Holland, Amsterdam and America Elsevier, New York.
* N. Catsimpoolas (1976), "Methods of protein separation." Plenum Press, New York.
* P.D. Allen, E.A. Hill, A.E. Stokes (1977). "Plasma proteins. Analytical and preparative techniques." Blackwell Scientific, Oxford.
* T. Kremer, L. Boross (1979) " Gel Chromatography. Theory, methodology , applications." John Wiley & Sons Akademiai Kiado, Budapest.
* J.M. Curling (1980). " Methods of plasma protein fractionation." Academic Press, London.
* J. Woodward (1985). "Immobilized cells and enzymes. A practical approach ." IRL Press, Oxford.
* P.D.G. Dean W.S. Johnson, F.A. Middle (1985) "Affinity chromatography. A Practical Approach." IRL Press Oxford.
* B.D. Hames D. Rickwood (1987). "Gel electrophoresis of proteins. A practical approach." IRL Press, Oxford.
* D. Rickwood, B.D. Hames (1987). "Gel electrophoresis of nucleic acids. A practical approach." IRL Press, Oxford.
SPECIFICATIONS
SeaQualite SQA-01100
|
SeaQualite SQA-02100
| |
|
LOW EEO |
MEDIUM EEO | |
| Electroendosmosis(EEO) (1) |
0.09-0.130 |
0.16-0.19 |
| Moisture |
<7.0% |
<7.0% |
| Ash |
<0.5% |
<0.5% |
| Sulfate |
<0.20% |
<0.25% |
| Gel strength (1% gel) |
>1,200 gr/cm2 |
>1,000 gr/cm2 |
| Gel strength (1.5% gel) |
>2,500 gr/cm2 |
>2,000 gr/cm2 |
| Gel Point (1.5% gel) |
36 ± 1.5°C |
36 ± 1.5°C |
| Melting point (1.5% gel) |
88 ± 1.5°C |
88 ± 1.5°C |
| Turbidity (1% solution) (2) |
<40 NP |
<40 NP |
SeaQualite SQA-03025
|
SeaQualite SQA-04025
| |
|
HIGH EEO |
SPECIAL EEO | |
| Electroendosmosis(EEO) (1) |
0.23-0.26 |
>0.3 |
| Moisture |
<7.0% |
<7.0% |
| Ash |
<1.1% |
<1.5% |
| Sulfate |
<0.25% |
<0.30% |
| Gel strength (1% gel) |
>750 gr/cm2 |
>700 gr/cm2 |
| Gel strength (1.5% gel) |
>1,000 gr/cm2 |
>1,100 gr/cm2 |
| Gel Point (1.5% gel) |
36 ± 1.5°C |
36 ± 1.5°C |
| Melting point (1.5% gel) |
88 ± 1.5°C |
88 ± 1.5°C |
| Turbidity (1% solution) (2) |
<40 NP |
<40 NP |
(1) Wieme method pH 8.4
(2) Nephocolorimeter Coleman Model 9
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©Copyright 2001 Sooner Scientific Inc. All Rights Reserved
Ordering/Price Information - Please see page 47 or the index.
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