SeaQualite Agarose

SeaQualite SQA-06025 and SQA-07025 AGAROSE

These agaroses have a higher gelification point than SeaQualite SQA-01100, SQA-02100, SQA-03025, SQA-04025 and SQA-05100 types which give higher thermal stabilty to the gels. Another outstanding feature of these agaroses is the highly flexible and elastic nature of the gel set.

SeaQualite SQA-06025: This agarose, aside from being recommended for the same applications as SeaQualite SQA-01100, is especially recommended for the preparation of agarose beads due to its high mechanical resistance.

Suitable for use in protein electrophoresis such as counterelectrophoresis and crossed immunoelectrophoresis.

SeaQualite SQA-07025: This agarose is characterized by a very low viscosity which recommends its use for preparation of agarose beads, especially at very high concentrations.

For electrophoretic uses, SeaQualite SQA-06025 and SQA-07025 is an agarose with mechanical properties that enable reliable handling of the gel with minimum breakage due to the excellent elasticity of the product. The gel is so flexible that it withstands harsh manipulation in transfer from gelling to dyeing operations. In the preparation of agarose beads for chromatography production, SeaQualite SQA-07025 provides the beads with enhanced resistance, increasing their functionality with respect to ease in operations and increased yields.

Agarose SeaQualite SQA-06025 and SQA-07025, when applied in nucleic acids and especially, in lipoprotein fraction electrophoresis, exhibits a superior performance. Agarose SeaQualite SQA-06025 and SQA-07025 is recommended for the preparation of agarose beads used in chromatography, in general, and for bioreactor enzymatic support, in particular.

USES In Biochemistry for analytical and preparative separation techniques such as: DIFFUSION AND IMMUNODIFFUSION.

Electrophoresis: For protein and nucleic acids, their fractions and other particles having an electrical charge.

IMMUNOELECTROPHORESIS: For techniques such as counterelectrophoresis and electroimmunodiffusion Laurell Rocket technique).

AGAROSE BEADS PREPARATION: For Gel Permeation and Affinity Chromatography. For these uses, the type indicated is SeaQualite SQA-06025 and SQA-07025, low viscosity.

CELL CULTURE: Semi-solid media preparations for cell cultivation and cloning

ADVANTAGES

Our experience of working with different types of seaweed all over the world has enabled us to obtain unequaled batch to batch consistency.

ELECTROPHORESIS

Our Agarose SeaQualite SQA-06025 and SQA-07025 also offers the following advantages:

1. Much better elasticity when compared to agarose SeaQualite SQA-01100, SQA-02100, SQA-03025, SQA-04025 and SQA-05100.

2. Also in relation to SeaQualite SQA-01100, SQA-02100, SQA-03025, SQA-04025 and SQA-05100 a higher gel point is possible at equal concentrations.

3. Formulated to provide greater capacity for derivation and crossed-linking, when compared to other agaroses. Easy derivation to activate gels, which allows coupling of enzymes, antigens, antibodies and other substances to the gel structure. Chemical cross-linking of the gel structure. Chemical cross-linking of the gel produces a gel with exceptional mechanical properties as well as thermal stability. The resulting cross-linked gel can be sterilized by autoclaving.

4. Extraordinary mechanical resistance for more reliable and easier handling.

5. Possibility of varying pore size in accordance with particle size by modifying gel concentration, improving resolution in different electrophoretic processes.

6. Easy preparation of the gel consequence of its excellent solubility in a broad range of gel concentrations, obtained by simple dilution in aqueous buffers, either by standard boiling or by using a microwave oven.

7. Greater thermal stability derived from the very high gellifying hysteresis. (Hysteresis, the difference between gelling and melting temperatures.)

8. Enhanced fraction identification and quantification as a result of the excellent transparency of the gels both in visible and ultraviolet regions.

9. A higher gel point, simplifying the preparation of electrophoresis plates and agarose beads. In some cases immunodiffusion (the addition to the solution of antigens, antibodies and other sensitive proteins) or when cell and enzyme immobilization are necessary the uses of agaroses SeaQualite SQA-01100, SQA-02100, SQA-03025, SQA-04025 and SQA-05100 would be preferable.

10. Better behavior in some types of electrophoresis (e.g. DNA) where exceptionally low electroendosmosis values are required.

11. Exceptionally low absorption of fluorescent stains, promoting efficient washing to eliminate excess staining reagents.

12. Absence of toxicity thus avoiding neurotoxicity associated with polyacrylamide.

CHROMATOGRAPHY

SeaQualite SQA-06025 and SQA-07025 is highly recommended for agarose bead manufacturing because it has the following advantages:

1. Enhanced mechanical resistance due to its high gel strength which provides improved flow capacities in chromatographic column applications.

2. Due to its methoxylation pattern with increased and specifically located CH3 groups, the beads have improved capacities to function in diphasic liquids (aqueous /organic solvent emulsions), resulting in increased operational yields.

3. Beads resist stirring without breakage due to a molecular structure that protects them when used as enzymatic support in applications that employ bioreactors.

AGAROSE EQUIVALENCE CHART

Sooner Scientific

BMA

Sigma

Bio-Rad

Gibco

Promega

Fisher

SeaQualite SQA-06025 SeaKem HGT A3768, A7174 162-0102 No Equivalence No Equivalence BP-164

SeaQualite SQA-07025 No equivalence A7299 No Equivalence No Equivalence No Equivalence No Equivalence

BIBLIOGRAPHY

* R.J. Wieme (1965). "Agar gel electrophoresis". Elsevier, Amsterdam.

* H. Determan (1969). "Chromatographie sur gel". Masson et Cie., Paris.

* L. Fisher (1974). "An introduction to gel chromatography". North Holland, Amsterdam and American Elsevier, New York.

* N. Catsimpoolas (1976), "Methods of protein separation ". Plenum Press, New York.

* P.D. Allen, E.A. Hill, A.E. Stokes (1977). "Plasma protein. Analytical and preparative techniques." Blackwell Scientific, Oxford.

* T. Kremer, L. Boross (1979) " Gel Chromatography. Theory, methodology , applications." John Wiley & Sons Akademiai Kiado, Budapest.

* J.M. Curling (1980). " Methods of plasma protein fractionation." Academic Press, London.

* J. Woodward (1985). "Immobilized cells and enzymes. A practical approach ." IRL Press, Oxford.

* P.D.G. Dean W.S. Johnson, F.A. Middle (1985) "Affinity chromatography. A Practical Approach." IRL Press Oxford.

* B.D. Hames D. Rickwood (1987). "Gel electrophoresis of proteins. A practical approach." IRL Press, Oxford.

* D. Rickwood, B.D. Hames (1987). "Gel eletrophoresis of nucleic acids. A practical approach." IRL Press, Oxford.

SPECIFICATIONS:

  
         
      SeaQualite SQA-06025

       

      SeaQualite SQA-07025

       
 STD VISCOSITY  LOW VISCOSITY
Electroendosmosis
      PH 8.4(EEO) (1) <0.14 <0.14
Moisture 
      <10.0% 
      <10.0%
Ash  
      <0.6% 
      <0.6%
Sulfate 
      <0.20% 
      <0.20%
Gel strength (1% gel)  
      >900 gr/cm2 
      >500 gr/cm2
Gel strength
      (1.5% gel) 
      >1,200 gr/cm2 
      >900 gr/cm2
Gel Point (1.5%
      gel)  
      41 ± 1.5°C 
      41 ± 1.5°C
Melting
      point (1.5% gel) 
      87 ± 1.5°C 
      87 ± 1.5°C
Turbidity
      (1% solution)  
      <50 NP 
      <50 NP
VISCOSITY (10% LOL
      80 °C) (2)

(1) Wieme method

(1) 10% = Actual concentration, humidity deducted.

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Ordering/Price Information - Please see page 47 or the index.

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Sooner Scientific	BMA	Sigma	Bio-Rad	Gibco	Promega	Fisher
SeaQualite SQA-06025	SeaKem HGT	A3768, A7174	162-0102	No Equivalence	No Equivalence	BP-164
SeaQualite SQA-07025	No equivalence	A7299	No Equivalence	No Equivalence	No Equivalence	No Equivalence

	SeaQualite SQA-06025	SeaQualite SQA-07025
	STD VISCOSITY	LOW VISCOSITY
Electroendosmosis PH 8.4(EEO) (1)	<0.14	<0.14
Moisture	<10.0%	<10.0%
Ash	<0.6%	<0.6%
Sulfate	<0.20%	<0.20%
Gel strength (1% gel)	>900 gr/cm2	>500 gr/cm2
Gel strength (1.5% gel)	>1,200 gr/cm2	>900 gr/cm2
Gel Point (1.5% gel)	41 ± 1.5°C	41 ± 1.5°C
Melting point (1.5% gel)	87 ± 1.5°C	87 ± 1.5°C
Turbidity (1% solution)	<50 NP	<50 NP
VISCOSITY (10% LOL 80 °C) (2)