Sooner Scientific Catalog
Sooner Scientific Catalog
P.O. Box 180 - Garvin, OK 74736 - 580-286-7047
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SeaQualite SQA-08025 AGAROSE:
This agarose is characterized by very high gel strength, which allows for use at very low concentrations (0.5%), therefore, facilitating the separation of very large DNA fragments such as chromosomes.
Changing concentrations can enable separations of DNA sizes from 1000bp-1.8Mb.
-Suitable for analytical gels using conventional constant field electrophoresis and especially recommended for Pulsed Field Gel Electrophoresis (PFGE).
-No detectable DNase or RNase activity.
-Due to its unique molecular structure, its dissolution requires the use of an autoclave. This form of dissolution will not however, produce subsequent alterations to the mechanical properties of the gel or its separation capabilities.
-The fact its dissolution is effected in an autoclave makes this agarose highly recommended for precast gels which need to be sterilized to avoid bacterial deterioration and are designed for long storage periods at room temperature.
A neutral polysaccharide with a very high molecular weight, much higher than other agaroses, that provides extremely high gel strength. Gel structure is similar to our agarose SeaQualite SQA-01100, SQA-02100, SQA-03025, SQA-04025 and SQA-05100 (medium temperature agarose) with an exclusion limit that is also similar.
Agarose SeaQualite SQA-08025 is obtained from seaweed beds that have been monitored for several years to assure that reproduction of the plants has taken place with almost no genetic variations. Harvesting is done when molecular weight is at its peak and sulfate content is lowest.
Dissolution requires boiling under reflux conditions; the standard method of heating in a microwave oven during two minutes may not be adequate. It is best to dissolve Agarose SeaQualite SQA-08025 in an autoclave for ten minutes at 121 °C.
USES In Biochemistry and Molecular Biology, for analytical and preparative separation techniques.
Conventional Electrophoresis: Suitable for protein, nucleic acids and macromolecules having an electrical charge. Can be used with a broad range of particle sizes just by altering gel concentration.
Pulsed Field Electrophoresis: The high exclusion limits achieved with lower gel concentrations allow the passage in less time of DNA fragments with sizes over 0.5 Mb.
Agarose Bead Preparation: Opens up new possibilities for agarose bead applications. Due to its extremely high gel strength, higher compressibility is achieved permitting greater flow of liquids without damage to the beads.
Cell and Enzyme Immobilization: For use as a catalytic bed for enzyme reactors. Owing to its greater gel strength, it is able to withstand tougher operating conditions.
Precast Electrophoresis Gels: In the preparation of precast gels, Agarose SeaQualite SQA-08025 can be dissolved by autoclaving.
ADVANTAGES
Our experience of working with different types of seaweed all over the world has enabled us to obtain greater yields and unequaled batch to batch consistency.
Agarose SeaQualite SQA-08025 offers the following advantages.
1. Extraordinary mechanical resistance for more reliable and easier handling.
2. Possibility of varying pore size in accordance with particle size by modifying the gel concentration, thus improving resolution in different electrophoretic processes.
3. Extremely high gel strength allowing for lower gel concentrations, enabling the product to be used not only with high molecular weight nucleic acids, including certain chromosomes, but also with large-sized particles like viruses and ribosomes.
4. Greater thermal stability derived from the very high gellifying hysteresis. (Hysteresis, the difference between gelling and melting temperature.)
5. Enhanced fraction identification and quantification as a result of the excellent transparency of the gels, both in visible and ultraviolet regions.
6. Exceptionally low absorption of florescent stains, promoting efficient washing to eliminate excess staining reagents.
7. Easy derivatization to activate gels, permitting coupling of enzymes, antigens, antibodies and other substances of the gel structure. Chemical cross-linking of the gel produces a gel with exceptional mechanical properties as well as thermal stability. The cross-linked gel can be autoclaved for sterilization.
8. Absence of toxicity thus avoiding neurotoxicity caused by polyacrylamide.
SPECIFICATIONS:
|
SeaQualite SQA-08025 |
|
| Electroendosmosis(EEO) (1) |
<0.135 |
| Moisture |
<7.0% |
| Ash |
<0.4% |
| Sulfate |
<0.10% |
| Gel strength (1% gel)(2) |
>1600 gr/cm2 |
| Gel strength (1.5% gel) |
>3000 gr/cm2 |
| Gelling Point (1.5% gel) |
36 ± 1.5 °C |
| Melting point (1.5% gel) |
88 ± 2.0 °C |
| Turbidity 1% (3) |
<40 NP |
(1) Wieme method pH 8.4
(2) Cherry Burrel
(3) Nephocolorimeter Coleman Model 9
AGAROSE EQUIVALENCE CHART
Sooner Scientific |
BMA |
Sigma |
Bio-Rad |
Gibco |
Promega |
Fisher |
| SeaQualite SQA-08025 | No equivalence | No equivalence | 162-0137,-0138 | No Equivalence | No Equivalence | No Equivalence |
BIBLIOGRAPHY
* R.J. Wieme (1965). "Agar gel electrophoresis". Elsevier, Amsterdam.
* H. Determan (1969). "Chromatographie sur gel". Masson et Cie., Paris.
* L. Fisher (1974) "An introduction to gel chromatography". North Holland, Amsterdam and American Elsevier, New York.
* N. Catsimpoolas (1976), "Methods of protein separation ." Plenum Press, New York.
* P.D. Allen, E.A. Hill, A.E. Stokes (1977). "Plasma protein. Analytical and preparative techniques." Blackwell Scientific, Oxford.
* T. Kremer, L. Boross (1979) " Gel Chromatography. Theory, methodology , applications." John Wiley & Sons Akademiai Kiado, Budapest.
* J.M. Curling (1980). " Methods of plasma protein fractionation." Academic Press, London.
* D.C. Schwartz, C.R. Cantor (1984). "Separation of yeast-sized DNAs by Pulse Field Gel Electrophoresis". Cell vol. 37.
* J. Woodward (1985). "Immobilized cells and enzymes. A practical approach ." IRL Press, Oxford.
* P.D.G. Dean W.S. Johnson, F.A. Middle (1985) "Affinity chromatography. A Practical Approach." IRL Press Oxford.
* G.F. Carle, M. Frank, M. Olson (1986). "Electrophoretic separations of large DNA molecules by periodic inversion of the electric field." Science, vol. 232.
* B.D. Hames D. Rickwood (1987). "Gel electrophoresis of proteins. A practical approach." IRL Press, Oxford.
* D. Rickwood, B.D. Hames (1987). "Gel eletrophoresis of nucleic acids. A practical approach." IRL Press, Oxford.
* P. Serwer (1988). "The mechanism of DNA's fractionation during Pulsed Field Agarose Gel Electrophoresis. A hypothesis". Applied and Theoretical Electrophoresis 1.
* E. Lai, B.W. Birren, S.M. Clark, J. Simon, L Hood (1989). "Pulsed Field Gel Electrophoresis." Biotechniques, vol 7.
* S. Ferris, S. Freeby, P. Zoller et al (1989). "A megabase DNA electrophoresis system." IBL
* P. Serwer (1990). "Sieving by agarose gels and its use during pulsed field electrophoresis." Biotechnology and Genetic Engineering Reviews, vol. 8.
* D.L. Holmes, N.C. Shellwagen (1990). "The electric field dependence of DNA mobilities in agarose gels: a reinvestigation". Electrophoresis, 11.
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