Sooner Scientific Catalog

P.O. Box 180 - Garvin, OK 74736 - 580-286-7047

| Catalog Index | Price Index | E-mail Us |

SeaQualite Agarose

SeaQualite SQA-09025 AGAROSE:

This high gel strength agarose is especially designed for a wide range of molecular biology techniques from conventional constant field to Pulsed Field Gel Electrophoresis (PFGE).

-Dissolves easily in a microwave.

-Has great versatility at working concentrations of (0.4-2%) in all buffer systems.

-DNA fragments from 50kb to 200bp may be separated through conventional electrophoresis.

-Can Reduce electrophoresis times considerably and improve the separation of very large DNA fragments.

-No detectable DNase or RNase activity.

-Suitable for blotting assays.

A neutral polysaccharide suitable for separating a wide range of particle sizes by varying pore size through changes in gel concentration. Structurally, Agarose SeaQualite SQA-09025 is a linear polymer with a high molecular weight.

Because the nature of the product is different, the resulting gel structure is unlike those of traditional agaroses. This characteristic, added to the very low content in sulfates, produces an inter-catenary reaction that is very compact, leading to a gel of very high strength with a higher exclusion limit. The extremely high gel strength allows for lower gel concentrations (0.3%), enabling it to be used not only with high molecular weight nucleic acids including chromosomes, but also with large sized particles like viruses and ribosomes. High electrophoretic mobility of DNA is greater when compared to SQA-01100. Electrophoresis times are reduced depending upon buffer and agarose concentration used. Gel preparation is easy by simple dissolution in aqueous buffers either by standard boiling or by using a microwave oven. Greater thermal stability derived from the very high gellifing hystersis (difference between gelling and melting temperatures). Exceptionally low absorption of staining agents. Absence of toxicity.

USES in Biochemistry and Molecular Biology, for analytical and preparative separation techniques.

Conventional Electrophoresis: Suitable for protein, nucleic acids and macromolecules having an electrical charge. Can be used with a broad range of particle sizes just by altering gel concentration.

Pulsed Field Electrophoresis: Because of the more compact gel structure, higher exclusion limits can be achieved with the same concentrations. Lower gel concentrations also give high exclusion limits, allowing the passage in less time of DNA fragments larger than 0.5Mb.

Agarose Bead Preparation: Opens up new possibilities for agarose bead applications. The larger gel network allows the fixation of larger particles. Due to its extremely high gel strength, a greater flow of liquids can be obtained without damage to the beads.

Cell and Enzyme Immobilization: For use as a catalytic bed for enzyme reactors. Due to its greater gel strength, it is able to withstand tougher operating conditions.

ADVANTAGES

Our experience of working with different types of seaweed all over the world has enabled us to obtain greater yields and unequaled batch to batch consistency.

Agarose SeaQualite SQA-09025 offers the following advantages.

1. Extraordinary mechanical resistance for more reliable and easier handling.

2. Higher exclusion limits at identical gel concentrations.

3. Easy preparation of the gel as consequence of its excellent solubility in a broad range of gel concentrations, obtained by simple dilution in aqueous buffers either by standard boiling or by using a microwave oven.

4. Possibility of varying pore size in accordance with particle size by modifying the gel concentration, thus improving resolution in different electrophoretic processes.

5. Extremely high gel strength allowing for lower gel concentrations, enabling the product to be used not only with high molecular weight nucleic acids, including certain chromosomes, but also with large-sized particles like viruses and ribosomes.

6. Greater thermal stability derived from the very high gellifying hysteresis. (Hysteresis, the difference between gelling and melting temperature.)

7. Enhanced fraction identification and quantification as a result of the excellent transparency of the gels, in both the visible and ultraviolet regions.

8. Exceptionally low absorption of florescent stains, promoting efficient washing to eliminate excess staining reagents.

9. Formulated to provide greater capacity for derivatization and cross-linking, when compared to other agaroses. Easy derivatization to activate gels, permitting coupling of enzymes, antigens, antibodies and other substances of the gel structure. Chemical cross-linking of the gel can be sterilized by autoclave.

 

APPROPRIATE AGAROSE CONCENTRATION FOR DNA SIZES

SeaQualite Agarose
 1X TAE BUFFER  Gel Concentration  1X TBE BUFFER
 Size Range (bp)  %  Size Range (bp)

SQA-09025  
40000-3000 0.3 20000-2000

22000-2000

0.5

12000-1500

15000-1000

0.8

9000-1000

10000-200

1.0

6000-500

5000-200

1.8

3000-200

 

SPECIFICATIONS:
 
    

      
SeaQualite SQA-09025


      
 

Electroendosmosis(EEO) (1)
      
<0.120
 
Moisture
      
<7.0%
Ash
      
<0.25%
Sulfate 
      
<0.12%
Gel strength (1% gel)(2)
      
>1800 gr/cm2
Gel strength
      (1.5% gel)
      
>3200 gr/cm2
 Gelling Point
      (1.5% gel)
      
36 ± 1.5 °C
Melting
      point (1.5% gel)
      
88 ± 2.0 °C
Turbidity
      1% (3)
      
<40 NP

(1) Wieme method pH 8.4

(2) Cherry Burrel

(3) Nephocolorimeter Coleman Model 9

AGAROSE EQUIVALENCE CHART

Sooner Scientific
 
BMA
 
Sigma
 
Bio-Rad
 
Gibco
 
Promega
 
Fisher
SeaQualite SQA-09025 SeaKem Gold A0576, A2929 162-0135,-0136 No Equivalence No Equivalence No Equivalence

BIBLIOGRAPHY

* R.J. Wieme (1965). "Agar gel electrophoresis." Elsevier, Amsterdam.

* H. Determan (1969). "Chromatographie sur gel." Masson et Cie., Paris.

* L. Fisher (1974). "An introduction to gel chromatography." North Holland, Amsterdam and American Elsevier, New York.

* T. Kremer, L. Boross (1979) " Gel Chromatography. Theory, methodology , applications." John Wiley & Sons Akademiai Kiado, Budapest.

* D.C. Schwartz, C.R. Cantor (1984). "Separation of yeast chromosoma-sized DNAs by Pulse Field Gel Electrophoresis." Cell vol. 37.

* J. Woodward (1985). "Immobilized cells and enzymes. A practical approach ." IRL Press, Oxford.

* P.D.G. Dean W.S. Johnson, F.A. Middle (1985) "Affinity chromatography. A

Practical Approach." IRL Press Oxford.

* G.F. Carle, M. Frank, M. Olson (1986). "Electrophoretic separations of large DNA molecules by periodic inversion of the electric field." Science, vol. 232.

* B.D. Hames D. Rickwood (1987). "Gel electrophoresis of proteins. A practical approach." IRL Press, Oxford.

* D. Rickwood, B.D. Hames (1987). "Gel eletrophoresis of nucleic acids. A practical approach." IRL Press, Oxford.

* P. Serwer (1988). "The mechanism of DNA's fractionation during Pulsed Field Agarose Gel Electrophoresis. A hypothesis." Applied and Theoretical Electrophoresis 1.

* E. Lai, B.W. Birren, S.M. Clark, J. Simon, L Hood (1989). "Pulsed Field Gel Electrophoresis." Biotechniques, vol 7.

* S. Ferris, S. Freeby, P. Zoller et al (1989). "A megabase DNA electrophoresis system." IBL

* P. Serwer (1990). "Sieving by agarose gels and its use during pulsed field electrophoresis." Biotechnology and Genetic Engineering Reviews, vol. 8.

* D.L. Holmes, N.C. Shellwagen (1990). "The electric field dependence of DNA mobilities in agarose gels: a reinvestigation." Electrophoresis, 11.

 

 

All trademarks are the property of their respective owners.

©Copyright 2001 Sooner Scientific Inc. All Rights Reserved

 

Ordering/Price Information - Please see page 47 or the index.

<-previous page | next page->

| Home | Information | What's New | E-mail Us |