Sooner Scientific Catalog

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SeaQualite Low Melting Agarose

SeaQualite SQA-10025, SQA-11025, SQA-12100, SQA-13025, AND SQA-14025 are low melting agaroses derivatized by organic synthesis that offer the user lower temperatures and important reductions in both gelling and melting points. Essentially, the process generates methoxylates from the basic agarose structure.

Low melting agaroses are very useful in operations in the laboratory that involve cell and the tissue cultures from animal or plant sources. The lower gelling and melting temperatures enable the correct digestion of DNA and RNA in the melting gel, with efficient recovery of the particles without running the risk of denaturization.

ADVANTAGES

1. Highest gel strength in the market, 80% superior to existing products. This substantially improves reliability in handling; the gel does not break easily.

2. Excellent digestibility of DNA and RNA because of the absence of inhibitors.

3. Post-extraction purification of DNA and RNA is not a requirement.

4. Better separation and increased recovery rates of DNA and RNA fragments.

LOW MELTING AND LOW GELLING POINT AGAROSES GENERAL CHARACTERISTICS OF LOW MELT TYPES:

- Higher clarity (gel transparency) than standard melting and gelling temperature agaroses.

- Lower melting and gelling temperatures.

- And, like all low melting and gelling point agaroses in the market, they have a lower gel strength than standard melting agaroses.

SeaQualite SQA-10025, SQA-11025, SQA-12100, SQA-13025, AND SQA-14025: These agaroses have a greater sieving properties than standard melting agaroses and are recommended for separation of DNA/RNA fragments greater than 1000bp as well as proteins in preparative electrophoresis.

-The low melting temperature(<65 °C) allows for recovery, without damage, of nucleic acids below there denaturing temperatures.

-The low gelling temperature(24 °C-28 °C) assures the agarose will be in a liquid state at a temperature range where in-gel manipulations (such as enzyme digestion, ligation, DNA labeling, nick translation, random priming, PCR, etc.), can be performed without prior purification of the DNA from the gel slice.

-Tissue culture and viral plaque applications.

-No detectable DNase or RNase activity.

-May be used with all buffer systems at normal concentrations (1%).

 

APPROPRIATE AGAROSE CONCENTRATION FOR DNA SIZES

SeaQualite Agarose

1X TAE BUFFER

Gel Concentration

1X TBE BUFFER

Size Range (bp)

%

Size Range (bp)

SQA-10025

20000-500

0.75

12000-500

16000-300

1.0

8000-500

10000-250

1.25

4000-200

5000-200

1.50

3000-150

2500-100

1.75

2000-100

1500-50

2.0

1000-50

 

SeaQualite 11025 agarose is a low melting temperature agarose with the highest resolving capacity for large DNA fragments, >1000bp, including PCR products.This agarose is genetic quality tested and certified. This ensures the in-gel applications can be performed in remelted agarose, avoiding difficult DNA extraction steps. SeaQualite SQA-11025 agarose is ideal for digestion by agarose enzymes, which makes it very easy to recover large DNA fragments suitable for cloning or enzymatic processing.

Applications:

-Electrophoresis of DNA fragments >1000bp.

-In-gel enzymatic processing (digestion, ligation, PCR.).

-Preparative electrophoresis.

-Analysis and recovery of large DNA fragments for further applications.

Functional Test:

-DNA resolution: bands appear sharp and finely resolved.

-DNAse/RNAse activity: non detected.

-DNA binding: none detected.

-In-gel enzymatic degradation by agarose: passes test

-Gel background: very low after EtBr staining.

 

APPROPRIATE AGAROSE CONCENTRATION FOR DNA SIZES

SeaQualite Agarose
1X TAE BUFFER Gel Concentration 1X TBE BUFFER
Size Range (bp) % Size Range (bp)
SQA-11025
20000-500 0.75 12000-500

16000-300

1.0

8000-500

10000-250

1.25

4000-200

5000-200

1.50

3000-150

2500-100

1.75

2000-100

1500-50

2.0

1000-50

SeaQualite SQA-12100 agarose is a low melting temperature agarose with the highest resolving capacity for DNA fragments smaller than 1000 bp, especially PCR products ranging from 200 to 800 bp. This agarose is genetic quality tested and cedrtified. This ensures that in-gel applications can be performed in remelted agarose, avoiding difficult DNA extraction steps. SeaQualite SQA-12100 agarose is ideal for digestion by agarose enzymes, making it very easy to recover small DNA fragments suitable for cloning or enzymatic processing. SeaQualite SQA-12100 agarose can be used at high concentrations, forming gels with excellent clarity and a higher sieving capacity than standard melting agaroses. Due to their high gel strength, SeaQualite SQA-12100 agarose gels are very easy to handle, even at concentrations as low as 2%.

Applications:

-Electrophoresis of DNA fragments <1000bp.

-In-gel enzymatic processing (digestion, ligation, PCR.).

-Preparative electrophoresis.

-Analysis and recovery of small DNA fragments for further applications.

Functional Test:

-DNA resolution: bands appear sharp and finely resolved.

-DNAse/RNAse activity: non detected.

-DNA binding: none detected.

-In-gel enzymatic processing: passes test.

-In-gel enzymatic degradation by agarose: passes test

-Gel background: very low after EtBr staining.

APPROPRIATE AGAROSE CONCENTRATION FOR DNA SIZES

SeaQualite Agarose
1X TAE BUFFER Gel Concentration 1X TBE BUFFER
Size Range (bp) % Size Range (bp)
SQA-12100
1500-500 2.0 1000-400

700-150

3.0

800-100

300-70

4.0

150-10

50-10

5.0

<30

 


    

  





-The difference between SeaQualite SQA-10025, SQA-11025, SQA-12100,
and SeaQualite SQA-13025, AND SQA-14025 lies in the fact that
SeaQualite SQA-13025, AND SQA-14025 has a more open structure,
which allows for a slightly faster migration.



-Due to its low gelling point (24°-28 °C) it is recommended
to place the gel for one hour at 4°C before use to assure
maximum resolution.



SeaQualite SQA-13025 LOW MELT



This agarose has their gelling points (<17°C)
and gel strengths lower than the standard melt types. They have
to be formulated at greater than 1.2% gel concentrations because
at lower concentrations, the gels of these agaroses are very fragile
and cannot be handled properly.



At the same gel concentrations, SQA-13025 agarose has more
sieving capacity than the low melt types SQA-10025, SQA-11025
and SQA-12100.



-Recommended for Capillary Electrophoresis, hybridoma and tissue
cells cloning, preparation of plant protoplasts, viral plaque
and anchorage independent growth assays.



- No detectable DNase and RNase activity.



-May be used with all buffer systems.



As the gelling temperature of this agarose is lower than room
temperature, it is necessary that gelling be carried out at 4°C
overnight.



SeaQualite SQA-14025



This unique agarose has the lowest gelling point (< 13°C)
currently available in the world and are recommended for tissue
and cell culture.



Especially recommended for Capillary Electrophoresis.



SPECIFICATIONS: 

  

    
    
      
SeaQualite SQA-10025

    		
    
      
SeaQualite SQA-11025

    		
  

  

    Electroendosmosis(EEO) (1) 
    
      
<0.12
 
    
      
<0.12

  
 
  

    Moisture 
    
      
<7.0%
 
    
      
<7.0%

  
 
  

    Ash 
    
      
<0.4%
 
    
      
<0.4%

  
 
  

    Sulfate 
    
      
<0.12%
 
    
      
<0.1%

  
 
  

    Gel strength (1.5% gel) 
    
      
>500 gr/cm2
 
    
      
(1%)>250 gr/cm2

  
 
  

    Gel Point (1.5% gel) 
    
      
24-28°C
 
    
      
24-28°C

  
 
  

    Melting point (1.5% gel) 
    
      
<65.5
 
    
      
<65.5°C

  
 
  

    Clarity (1% solution) (2) 
    
      
<40 NP
 
    
      
<40 NP

  
 





    

      
SeaQualite SQA-12025


      
 

Electroendosmosis(EEO) (1)
      
<0.120
 
Moisture
      
<7.0%
Ash
      
<0.25%
Sulfate 
      
<0.12%
Gel strength (1% gel)(2)
      
>1800 gr/cm2
Gel strength
      (1.5% gel)
      
>3200 gr/cm2
 Gelling Point
      (1.5% gel)
      
36 ± 1.5 °C
Melting
      point (1.5% gel)
      
88 ± 2.0 °C
Turbidity
      1% (3)
      
<40 NP
 


   

      
SeaQualite SQA-13025


      
 


      
SeaQualite SQA-14025


      
 

 
Electroendosmosis(EEO) (1)
      
<0.12

      
>0.10
Moisture
      
<7.0%

      
<7.0%
 
Ash
      
<0.35%

      
<1.0%
Sulfate
      
<0.14%

      
<0.35%
 
Gel strength (1.5%
      gel)
      
(2%)>400 gr/cm2

      
>300 gr/cm2
Gel Point (1.5%
      gel)
      
(1%) 8-16°C

      
<13°C
 
Melting point
      (1.5% gel)
      
(1%)<63°C

      
<60°C
Clarity (1% solution)
      (2)
      
<40 NP

      
<40 NP
 



(1) Wieme method pH 8.4



(2) Nephocolorimeter Coleman Model 9





 



AGAROSE EQUIVALENCE CHART




  

    
      
Sooner Scientific

    		
    
      
BMA

    		
    
      
Sigma

    		
    
      
Bio-Rad

    		
    
      
Gibco

    		
    
      
Promega

    		
    
      
Fisher

    		
  

  

    SeaQualite SQA-10025 
    SeaPlaque 
    A4018, A0701 
    162-0017,-0019 
    15517-014,022 
    Low Melt Grade 
    BP-165
  
 
  

    SeaQualite SQA-11025 
    SeaPlaque GTG 
    A9414 
    No Equivalence 
    No Equivalence 
    PrepGrade 1000bp 
    No Equivalence
  
 
  

    SeaQualite SQA-12100 
    NuSieve GTG 
    A2790 
    162-0144 
    No Equivalence 
    PrepGrade10-1000bp 
    BP-1360
  
 
  

    SeaQualite SQA-13025 
    SeaPrep 
    A5030, A2576 
    No Equivalence 
    No Equivalence 
    No Equivalence 
    No Equivalence
  
 
  

    SeaQualite SQA-14025 
    No equivalence 
    No equivalence 
    No Equivalence 
    No Equivalence 
    No Equivalence 
    No Equivalence
  
 




 



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©Copyright 2001 Sooner Scientific Inc. All Rights Reserved



 



Ordering/Price Information - Please see page
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