Sooner Scientific Catalog
Sooner Scientific Catalog
P.O. Box 180 - Garvin, OK 74736 - 580-286-7047
| Catalog Index | Price Index | E-mail Us |
MOLECULAR SIEVING GRADE: SeaQualite SQA-15025, SQA-16025 and SQA-17025
These agaroses achieve exquisite separation by molecular sieving. They are recommended for separating nucleic acids less than 1000bp, including PCR and forensic DNA identity products.
SeaQualite SQA-15025 AGAROSE
SeaQualite SQA-15025 Resolution of DNA fragments similar to gels made with polyacrylamide at concentrations of 8%. SeaQualite SQA-15025 gels are easier to prepare and are free of neurotoxic dangers inherent with polyacrylamide.
SeaQualite SQA-15025 offers excellent resolution of DNA fragments with less than 500 bp, especially the smaller primer-size fragments. Capable of clearly separating DNA fragments smaller than 50 bp with differences of 3 bp between them. Forms a very clear, transparent gel even at concentrations of 5% or higher. Efficient mechanical handling at all concentrations. With SeaQualite SQA-15025 agarose, the chances of gel breaking or cracking when handled are greatly minimized. Low DNA binding and low background fluorescence when using Ethidium Bromide. To achieve the best resolution of SeaQualite SQA-15025 agarose gels, they must be maintained at 4°C for 20 hours before they are used.
SeaQualite SQA-15025: This low melting point agarose is unique in the marketplace with very high transparency, even at high concentrations (5%), and the ability to separate nucleic acid fragments less than 10 bp.
-Especially recommended for analytical gels of DNA with less than 500 bp.
-Recommended for Capillary Electrophoresis.
-No detectable DNase or RNase activity.
-Low DNA binding.
-May be used with all buffer systems.
-Not recommended for blotting assays.
Due to its high viscosity at concentrations higher than 3% its dissolution is recommended by boiling water bath or autoclave. This will not alter the mechanical properties of the gel nor its sieving capacity. It is recommended to leave the gel at 4°C overnight for proper gelling process and resolution properties.
SPECIFICATIONS:
SeaQualite SQA-15025
| |
| Electroendosmosis(EEO) (1) |
<0.12 |
| Moisture |
<7.0% |
| Ash |
<0.3% |
| Sulfate |
<0.11% |
| Gel strength (3% gel)(2) |
>500 gr/cm2 |
| Gel strength (5% gel) |
>1000 gr/cm2 |
| Gelling Point (3% gel) |
24 - 31 °C |
| Melting Point (3% gel) |
< 75 °C |
(1) Wieme method pH 8.4; at 2% concentration
(2) The measurement is carried out at room temperature after maintaining the gel at a temperature of 4°C for 20 hours.
DISSOLVING SeaQualite SQA-15025 AGAROSE
Method 1: Microwave (recommended for <3% concentrations)
- Add buffer and stir bar to a flask 2-4 times the desired volume of the solution.
- Place flask on a magnetic stirrer and sprinkle the agarose powder into the buffer while stirring to prevent the formation of clumps.
- Remove the stir bar.
- Weigh the flask
- Place the flask in microwave (1250 watt) and heat power for 1-2 minutes.
- Gently swirl to resuspend any agarose particles not yet dissolved.
- Reheat at 5-10 second intervals or until the solution comes to a boil, leaving 10-15 seconds between each heating phase to allow any foam to settle. Continue until the agarose is dissolved.
- Replace the lost weight by adding warm distilled water and mixing the solution gently.
- Let solution cool at room temperature for 15-20 minutes or until it reaches a temperature of 50-60°C. (Alternatively the solution can be left in a heated bath at 55°C for over 30 minutes).
Method 2: Boiling Water Bath (recommended for all concentrations, especially 4%)
- Using a flask 2-4 times the desired solution volume, add buffer at room temperature and stir bar.
- Put the flask on a hot plate with magnetic stirrer and slowly sprinkle the agarose powder while stirring constantly to prevent the formation of agarose clumps.
- Weight the flask and solution before heating.
- Place the flask in the bath and bring to a boil while stirring constantly.
- Keep flask in the bath and continue heating for 15-20 minutes after starting to boil, or until the agarose is completely dissolved.
- Turn off stirring while keeping flask in bath for an additional 15 minutes.
- Replace the lost weight by adding warm distilled water and mixing the solution.
- Let the solution cool at room temperature for 15-20 minutes or until it reaches a temperature of 50-60°C. (Alternatively, the solution can be left in a heated bath at 55°C for over 30 minutes).
Method 3: Autoclave (recommended for all concentrations, especially >5%)
- Add buffer and stir bar to a flask 2-4 times the desired volume of the solution.
- Place flask on a magnetic stirrer and sprinkle the agarose powder into the buffer while stirring to prevent the formation of clumps.
- Heat 2 minutes in the microwave at medium power.
- Cover the mouth of the flask with aluminum foil, sealing the opening completely to prevent spillover.
- Autoclave at 121°C for 15 minutes.
- Allow to cool at room temperature until the solution reaches a temperature of 50-60°C.
(1) 700 Watts power.
SeaQualite SQA-16025 AGAROSE
SeaQualite SQA-16025 Molecular Biology Grade Agarose exhibits the following properties:
High resolution of PCR products and DNA fragments with low base pairs. Better handling because of a stronger gel structure. Improved clarity of the gel enhancing visibility. Another attribute that can be observed when using SeaQualite SQA-16025 is its excellent mechanical strength when used in smaller concentrations. With SeaQualite SQA-16025 agarose, the chances of gel breakage or cracking when handled are greatly minimized. Low DNA binding and low background fluorescence when using Ethidium Bromide.
To achieve the best resolution with SeaQualite SQA-16025 agarose gels, they must be chilled for 30 minutes at 4°C just before they are used.
SeaQualite SQA-16025: This agarose is an intermediate melting and gelling point type which has superior sieving characteristics.
-Recommended for analytical gels of DNA with lower than 1000bp, especially PCR products, small DNA fragments generated by restriction enzyme digestion, and DNA fragments used in mutation analysis.
-Working at 1.8-3% concentrations, fragments from 150-1000bp may be separated.
Increasing concentrations to 4.5% allows for fragments up to 50bp in size to be separated.
-Can be used in blotting assays with fragments less than 600bp.
-No detectable DNase and RNase activity.
-Low DNA binding.
-May be used with all buffer systems.
At concentrations higher than 3% dissolution is recommended by boiling water bath or in an autoclave.
This will not cause any variation in the mechanical properties of the gel nor in its sieving capacity.
It is recommended to keep the gel for 1 hour at 4°C before it is used to assure a total gelling process.
APPROPRIATE AGAROSE CONCENTRATION FOR DNA SIZES
|
SeaQualite Agarose |
||
|
1X TAE BUFFER |
Gel Concentration |
1X TBE BUFFER |
|
Size Range (bp) |
% |
Size Range (bp) |
|
SQA-16025 |
||
|
1500-100 |
2.0 |
1200-100 |
|
1000-50 |
3.0 |
700-40 |
|
500-20 |
4.0 |
500-50 |
|
300-10 |
5.0 |
<100 |
SPECIFICATIONS:
SeaQualite SQA-16025
| |
| Electroendosmosis(EEO) (1) |
<0.12 |
| Moisture |
<7.0% |
| Ash |
<0.35% |
| Sulfate |
<0.11% |
| Gel strength (1.5% gel) |
>500 gr/cm2 |
| Gel strength (3% gel) |
>1500 gr/cm2 |
| Gelling Point (1.5% gel) |
<30 °C |
| Gelling Point (3% gel) |
<35 °C |
| Melting Point (1.5% gel) |
<70 °C |
| Melting Point (3% gel) |
< 75 °C |
(1) Wieme method pH 8.4
SeaQualite SQA-17025: This agarose is a standard melting and gelling temperature type which forms high strength gels.
-Recommended for analytical gels of DNA/RNA. At 2-5% concentrations, it is capable of separating fragments from 100 to 1500 bp.
-May be used in blotting assays.
-No detectable DNase or RNase activity.
-May be used with all buffer systems.
-Low DNA binding.
The chemical structure of the polymers has been altered, producing gels with a tighter network capable of sieving DNA fragments with less than 1000bp, including PCR products.
SeaQualite SQA-17025 delivers much higher gel strength. At the same time, the gel is exceptionally firm, but, still flexible when handled, minimizing the danger of cracking or breaking when manipulated.
SeaQualite SQA-17025 has the same melting and gelling temperature as regular agaroses, allowing faster and easier preparation of gels. SeaQualite SQA-17025 also offers end users the possibility of achieving excellent resolution at concentrations of 1% or less.
SeaQualite SQA-17025 agarose is recommended for all analytical applications. Unlike similar products, however, it can also be employed when DNA is recovered for subsequent use in enzymatic procedures.
SeaQualite SQA-17025 is free of DNase and RNase. Other characteristics are low DNA binding, low background fluorescence when using Ethidium Bromide and good transference to membrane in Southern Blotting (DNA fragments of 154 - 2176 bp in gels with 4% concentration).
APPROPRIATE AGAROSE CONCENTRATION FOR DNA SIZES
|
SeaQualite Agarose |
||
|
1X TAE BUFFER |
Gel Concentration |
1X TBE BUFFER |
|
Size Range (bp) |
% |
Size Range (bp) |
|
SQA-17025 |
||
|
2500-700 |
2.0 |
1500-500 |
|
1200-500 |
3.0 |
800-100 |
|
700-100 |
4.0 |
500-50 |
|
250-30 |
5.0 |
250-20 |
SPECIFICATIONS:
|
SeaQualite SQA-17025 |
|
| Electroendosmosis(EEO) (1) |
<0.12 |
| Moisture |
<7.0% |
| Ash |
<0.35% |
| Sulfate |
<0.11% |
| Gel strength (1.5% gel) |
>2000 gr/cm2 |
| Gel strength (4% gel) |
>4250 gr/cm2 |
| Gelling Point (1.5% gel) |
36.5 °C |
| Gelling Point (4% gel) |
40.5 °C |
| Melting Point (1.5% gel) |
89 °C |
| Melting Point (4% gel) |
93 °C |
AGAROSE EQUIVALENCE CHART
Sooner Scientific |
BMA |
Sigma |
Bio-Rad |
Gibco |
Promega |
Fisher |
| SeaQualite SQA-15025 | No equivalence | No equivalence | No Equivalence | No Equivalence | No Equivalence | No Equivalence |
| SeaQualite SQA-16025 | Metaphor | A8455 | No Equivalence | 10975-027,035 | No Equivalence | No Equivalence |
| SeaQualite SQA-17025 | NuSieve 3:1 | A7431 | No Equivalence | No Equivalence | No Equivalence | No Equivalence |
All trademarks are the property of their respective owners.
©Copyright 2001 Sooner Scientific Inc. All Rights Reserved
Ordering/Price Information - Please see page 47 or the index.
<-previous page | next page->
| Home | Information | What's New | E-mail Us |