Sooner Scientific Catalog
Sooner Scientific Catalog
P.O. Box 180 - Garvin, OK 74736 - 405-237-0302
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SEAQUALITE AGAR SQA-20000 PURIFIED BACTERIOLOGICAL AGAR
This highly purified agar for use in Biochemistry and Microbiology is processed from carefully selected Gelidium seaweeds (Rhodophyceae). Through a purification process the product obtained has an ash content essentially lower than that of bacteriological agar. This process also has decreased the small oligosaccharides fraction and increased the agarose contents. The physicochemical, biochemical and bacteriological properties of this product are strictly controlled to guarantee its exceptional behavior in biochemical, bacteriological and mycological applications.
USES: in Biochemistry: Immunodiffusion: To be used as support
Electrophoresis: Excellent as support.
Cell Culture: Excellent for tissues and cell culture media.
Bacteriology: Essentially free of impurities that could interfer with other products in culture media formulation. Free of bacterial inhibitors. Recommended in media to be used for determination of the susceptibility of certain microorganisms or bacteriostatic drugs.
Mycology: For assimilation test in yeasts and molds. For susceptibility est for fungicidal and fungistatic drugs.
BIBLIOGRAPHY
* R.J. Wieme (1965). "Agar gel electrophoresis." Elsevier, Amsterdam.
* D. Freifelder. "Physical Biochemistry: Applications to Biochemistry and Molecular Biology." Freeman and Col. San Francisco.
SPECIFICATIONS:
PURIFIED AGAR SQA-20000
| |
| Electroendosmosis(EEO) (1) |
<0.450 |
| Moisture |
<10.0% |
| Ash |
<1.6% |
| Gel strength (1.5% - Nikkan) |
700-1200 gr/cm2 |
| Gelling Point (1.5% gel) |
<32 - 37.5 °C |
| Melting point (1.5% gel) |
<80 - 95 °C |
| Clarity 1.5% sol |
<100 NP |
| pH (1.5%) |
5.8-7.0 |
| Colorimetry (absorbance) 430 nm |
<0.150 |
(1) Wieme method pH 8.4
SEAQUALITE SQA-21000E AND SQA-21000A BACTERIOLOGICAL GRADE AGAR:
Bacteriological agar is a gelling agent used in the preparation of culture media and in other bacteriological applications.
The main advantage is the absence of inhibitors which could hinder optimal development of microorganisms. In addition, bacteriological agar also possesses other attributes such as transparency, high hysteresis and very reliable reproducibility.
Two types of bacteriological agar coexist in the market: European and American varieties. The first type has a higher gel strength and is used in concentrations of 1.0% to 1.2%, American-type agar gives lower gel strength and is utilized at concentrations ranging from 1.2% to 1.6%.
Each batch produced is thoroughly tested for biological performance against a battery of known bacterial cultures in order to ensure proper growth characteristics and the absence of inhibitors. Additional QC test are carried out to be certain that each batch meets established physical and chemical specifications.
BACTERIOLOGICAL GRADE AGAR
SPECIFICATIONS:
SeaQualite SQA-21000E |
SeaQualite SQA-21000A |
|
|
European Type |
American Type |
|
| Moisture |
<10.0% |
<10.0% |
| Ash | <4.5% |
<6.5% |
| Gel strength (1.5% - Nikkan) | 800-1100 gr/cm2 |
600-750 gr/cm2 |
| Gelling Point (1.5% gel) | 35°C ±3°C |
35°C ±3°C |
| Melting point (1.5% gel) | 85°C ±5°C |
85°C ±5°C |
| Clarity 1.5% (HACH) | < 12 NTU |
< 12 NTU |
| pH (1.5%) before autoclaving | 7.0 ±0.4 |
7.0 ±0.4 |
| pH (1.5%) after autoclaving | 6.5 ±0.4 |
6.5 ±0.4 |
| Colorimeter (absorbance) 430 nm | <0.200 |
<0.200 |
The particle size of both types is 95% pass mesh 60 ASTM
SQA-220000-1 Agar Technical Grade, Type B (40/60 Mesh) and SQA220000-2 Agar Technical Grade, Type B (80/100 Mesh)
General Description: Derived from Gelidium seaweeds
Primary Application: Use in microbiological and tissue culture media
Physical and Chemical Characteristics
Color: White to light cream
Particle Size: 40/60, 80/100 mesh sieve
Moisture: 20% or less
Gel Strength: 600-1100 g/cm2 (Nican, 1.5% sol
Melting Point: 82-88C (1.5% sol)
pH in Gel: 6.0- 8.0 ( 1.5% )
Turbidity: >50 NTU
Ash: 6.5% or less
Acid insoluble ash: 0.5% or less
Aresenic: less than 3 ppm
Lead: less than 5 ppm
Heavy metals: less than 40 ppm
Solubility: In boiling water
Microbiological Analysis:
Total plate count: <5000/g
Coliforms: < 3/g
E. Coli: Nil
Yeasts and molds: <100/g
APPROPRIATE AGAROSE CONCENTRATION FOR DNA SIZES
SeaQualite Agarose
1X TAE BUFFER Gel Concentration 1X TBE BUFFER Size Range (bp) % Size Range (bp) SQA-01100, SQA-05100 20000-1000 0.6 15000-1000 12000-500
0.8
10000-500
8000-300
1.0
7000-250
6000-200
1.2
5000-200
3500-100
1.5
2000-50
SQA-09025
|
40000-3000 |
0.3 |
20000-2000 |
|
22000-2000 |
0.5 |
12000-1500 |
|
15000-1000 |
0.8 |
9000-1000 |
|
10000-200 |
1.0 |
6000-500 |
|
5000-200 |
1.8 |
3000-200 |
SQA-16025
|
1500-100 |
2.0 |
1200-100 |
|
1000-50 |
3.0 |
700-40 |
|
500-20 |
4.0 |
500-50 |
|
300-10 |
5.0 |
<100 |
SQA-17025
|
2500-700 |
2.0 |
1500-500 |
|
1200-500 |
3.0 |
800-100 |
|
700-100 |
4.0 |
500-50 |
|
250-30 |
5.0 |
250-20 |
SQA-12100
|
1500-500 |
2.0 |
1000-400 |
|
700-150 |
3.0 |
800-100 |
|
300-70 |
4.0 |
150-10 |
|
50-10 |
5.0 |
<30 |
SQA-10025, SQA-11025
|
20000-500 |
0.75 | 12000-500 |
|
16000-300 |
1.0 | 8000-500 |
|
10000-250 |
1.25 | 4000-200 |
|
5000-200 |
1.50 | 3000-150 |
|
2500-100 |
1.75 | 2000-100 |
|
1500-50 |
2.0 | 1000-50 |
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©Copyright 2001 Sooner Scientific Inc. All Rights Reserved
Ordering/Price Information - Please see page 47 or the index.
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