Sooner Scientific Catalog

P.O. Box 180 - Garvin, OK 74736 - 580-286-7047 Fax 580-286-4268

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• Low Volume Sequencers, Single and Dual

Left: Single Low Volume Sequencer, 52cm (h ) x 33cm (w), Cat. # CG-502-33.
Right: Dual Low Volume Sequencers, 52cm (h) x 33cm (w), Cat. # CCG-502-33.

SINGLE LOW VOLUME SEQUENCER

The Sooner Scientific Low Volume Sequencers are available in 20cm and 33cm widths and can be ordered with a height of 42cm, 52cm, or 62cm. The reservoirs' low profile design requires less buffer to maintain buffer flow through the gel. Save expense by using as little as 250ml of buffer per reservoir for the 20cm wide units, and 400ml per reservoir for the 33cm units. This sequencer features a bar clamp, a built-in heat dispersion plate, flip-lids, and detachable upper and lower buffer reservoirs. The new hinged flip-lid safety covers allow easy access to the reservoirs and can never be misplaced.

Each Sequencer includes:

• Built-in epoxy-coated aluminum heat dispersion plate
• Bar clamp
• Leak-proof silicone gasket on upper reservoir
• Flip-lid safety covers
• 4 molded white spring clamps
• Safety-tip power leads
• Leveling base and bubble level
• Buffer drainage ports
• SeaQuate pre-mixed acrylamide/bis gel solution is shipped with each order. Ready to use fast and easy. SeaQuate comes to you in 1 pre-mixed bottle containing TEMED, Urea, TBE and acrylamide. Add APS and pour the gel. Choose One: SQL 4301, SQL 5301, SQL 6301 or SQL 8301.

DUAL LOW VOLUME SEQUENCER

The Sooner Scientific Dual Low Volume Sequencer has all of the convenience and safety features of our Single Low Volume Sequencer, but can run twice as many samples using less buffer and the same amount of valuable bench space. The Dual Low Volume Sequencer is available in 20cm and 33cm widths and with height capabilities of 42cm, 52cm, or 62cm. A rotating base allows the unit to swivel, providing easy access to either side. This sequencer features two bar clamps, built-in heat dispersion plates, flip-lids, and detachable upper and lower buffer reservoirs. The new hinged flip-lid safety covers allow easy access to the reservoirs and can never be misplaced.

Each Dual Sequencer includes:

• Built-in epoxy-coated aluminum heat dispersion plates
• 2 bar clamps
• Rotating base
• Leak-proof silicone gasket on upper reservoir
• Flip-lid safety covers
• 8 molded white spring clamps
• Safety-tip power leads
• Leveling base and bubble level
• Buffer drainage ports
• SeaQuate pre-mixed acrylamide/bis gel solution is shipped with each order. Ready to use fast and easy. SeaQuate comes to you in 1 pre-mixed bottle containing TEMED, Urea, TBE and acrylamide. Add APS and pour the gel. Choose One: SQL 4301, SQL 5301, SQL 6301 or SQL 8301.

SPECIAL FEATURES OF LOW VOLUME SEQUENCERS

• The Dual Sequencer upper reservoir is divided in half, allowing the researcher to run identical or independent buffer conditions on either side. This feature is also convenient for the researcher who occasionally wants to run just one side of the unit.
• The reservoirs' low profile design requires less buffer to maintain buffer flow through the gel. Save expense by using as little as 250ml of buffer per reservoir for the 20cm wide units, and 400ml per reservoir for the 33cm units.
• Flip-lids remain attached to chamber even when open, for convenience and ease of use. (Pictured at right).
• Convenient built-in, epoxy-coated aluminum plates help to evenly distribute the heat generated during electrophoresis, eliminating the "smile effect." These heat dispersion plates are anodized for electrical isolation and white epoxy-coated for easy visualization of tracking dyes. These aluminum plates are also spring-mounted to reduce stress when clamping on sequencing glass plates and to help provide a close and even contact with the back glass plate.
• Quick-disconnect tubing coupling with shut-off valves or ball valve with simple on-off lever can be ordered as alternatives to the tubing adapters, simplifying buffer disposal. (See here).
• The Dual Sequencer's rotating base allows unit to swivel providing easy access to either side of the unit. A removable peg locks the base of the unit into place, providing stability for either 180° rotation. Simply load one side, remove peg, rotate, replace peg, and continue loading alternate side.
• Upper and lower reservoirs may be removed from the body of the unit for ease in cleaning.
• Acrylic bar clamp provides equal pressure along entire width of the glass plate/comb sandwich eliminating capillary leakage of sample while loading sharktooth combs. This clamp minimizes glass plate fractures due to the "bending" that can occur from the uneven pressure of side clamps alone, resulting in longer plate life.

Shown above: Flip-lids in open position.

APPLICATIONS

• DNA Sequencing
• Differential Display
• Mutation Detection
• DNA Fingerprinting
• Genotyping
• DNA Footprinting
• SDS Page
• Microsatellites/SSTR
• Minisatellites
• RNase Protection

Accessories

Please click here for description of combs, spacers, glass plate sets, bar clamps, aluminum heat dispersion plates, sliding plate gel caster, white clamps, buffer disposal options, glass racks and gel tape.

Ordering/Price Information - Please see page 51 or the index.

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