Sooner Scientific Catalog

P.O. Box 180 - Garvin, OK 74736 - 580-286-7047 Fax 580-286-4268

| Home | E-mail Us |

 

SeaQuate DNA Sequencing Gel Solution

See How Easy DNA Sequencing Can Be!
With SeaQuate DNA sequencing Gel Solution you avoid mixing, measuring and mess because the solution comes to you in 1 premixed bottle containing TEMED, urea, TBE and acrylamide. Just add APS and pour. So from start to finish your gel is ready to run in 30 minutes.

Safe
The liquid format of Sooner Scientific's SeaQuate DNA Sequencing Solution eliminates the safety hazards of dry acrylamide.

Reproducible Results
Each lot of SeaQuate DNA Sequencing Gel Solution is first tested and qualified in the Sooner Scientific QC lab for conductivity, pH and refractive index. Then a test sequencing gel is run to ensure band sharpness and migration. All this adds confidence; confidence that you will get consistent, reproducible, quality results time after time.

 

Kit Contents:
1.0 L SeaQuate 6% (Cat. No. SQL6301) SeaQuate 8% (Cat. No. SQL6301) DNA Sequencing Gel Solution

 

Storage
For best performance keep frozen until opening. After opening store at 4C in the dark.

Stability
5 months in the dark, at 4C.

Preparation:

  1. Dilute 1.0g of APSwith 10ml of D.I. water. To bring the APS into solution, shake gently, avoid vigorous shaking as this will cause the solution to effervesce. Note: The 10% APS solution will remain stable for several weeks in the refrigerator.
  2. Sequencing gel solutions stored below 8C will cause the formation of urea crystals. Warming in a 30C water bath will bring the urea back into solution.
  3. Mix 0.1ml 10% APS solution (see step 1) for every 10ml of DNA Sequencing Gel Solution needed (e.g. for a 40ml gel, 0.4ml of 10% APS solution is required).
  4. Pour the gel. At 20C the gel should polymerize in less than 20 minutes.

References:

  1. Maniatas, T., Fritsch, E.F. and Sambrook, J. (1991), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 6.40.
  2. Maniatas, T., Fritsch, E.F. and Sambrook, J. (1982), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 185.

Automated DNA Sequencing Gel Solutions: Typical Electropherograms

SeaQuate SQL 4750: The gel solution choose by Washington University Genome Sequencing Center for use with the ABI 373S in mapping the Merck EST mapping project for the public data base. Electropherogram provided courtesy of Dr. Richard Wilson and Dr. Elaine Mardis Washington University Genome Seqiuencing Center St Louis, Missouri USA

Additional Examples
Photo 1 Photo 2

SeaQuate SQL 4250: 4.25% gel solution run with ABI 377. These gel electroperograms are provide courtsey of Dr. Bruce Roe of the University of Oklahoma Genome Sequencing Center, Chemistry Department, Norman, Oklahoma USA

Additional Examples
Photo 1 Photo 2 Photo 3
Photo 4 Photo 5 Photo 6

SeaQuate SQL 4750: 4.75% gel solution run with ABI 373S. These gel electroperograms are provide courtsey of Dr. Bruce Roe of the University of Oklahoma Genome Sequencing Center, Chemistry Department, Norman, Oklahoma USA

SeaQuate SQL 6500: 6.5% gel solution run with ABI 373A. This gel solutions yeilds better resolution and longer read length as compaired to the standard 6% gel solution normally used with the ABI 373. Electroperograms are provide courtsey of Dr. Donna Muzny and Dr. Richard Gibbs of the Baylor College of Medicine Genome Sequencing Center, Houston, Texas USA

Additional Examples
Photo 1 Photo 2A Photo 2B

SeaQuate SQL 5500: 5.5% gel solution run ABI 373 . Electropherogram provided courtesy of Dr. Christiana Robbins and Dr. Eric Green of the National Institute of Health, Natioanl Human Genome Sequencing Center, Bethesda, Maryland USA

Additional Examples
Photo 1 Photo 2
Photo 3 Photo 4

SeaQuate SQL 5500: 5.5% gel solution run ABI 377 . Electropherogram provided courtesy of Dr. Christiana Robbins and Dr. Eric Green of the National Institute of Health, National Human Genome Sequencing Center, Bethesda, Maryland USA

Additional Examples
Photo 1 Photo 2
Photo 3 Photo 4

SeaQuate SQL 4250: 4.25% gel solution run with ABI 377. Electropherogram provided courtesy of Dr. Christiana Robbins and Dr. Eric Green of the National Institute of Health, National Human Genome Sequencing Center, Bethesda, Maryland USA

Additional Examples
Photo 1 Photo 2 Photo 3
Photo 4 Photo 5 Photo 6
Photo 7 Photo 8 Photo 9
Photo 10 Photo 11 Photo 12
Photo 13 Photo 14 Photo 15
Photo 16 Photo 17 Photo 18
Photo 19 Photo 20 Photo 21
Photo 22 Photo 23 Photo 24
Photo 25 Photo 26 Photo 27
Photo 28 Photo 29 Photo 30
Photo 31 Photo 32 Photo 33
Photo 34 Photo 35 Photo 36
Photo 37 Photo 38 Photo 39
Photo 40 Photo 41 Photo 42
Photo 43 Photo 44 Photo 45
Photo 46 Photo 47 Photo 48
Photo 49 Photo 50 Photo 51
Photo 52 Photo 53 Photo 54
Photo 55 Photo 56 Photo 57

 

Ordering/Price Information - Please see Page 56 or the index.

<-previous page | next page->

| Home | Information | What's New | E-mail Us |