Sooner Scientific Inc.

Sooner Scientific Catalog

P.O. Box 180 - Garvin, OK 74736 - 580-286-7047 Fax 580-286-4268

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AGAR

SEAQUALITE AGAR SQA-20000 PURIFIED BACTERIOLOGICAL AGAR

This highly purified agar for use in Biochemistry and Microbiology is processed from carefully selected Gelidium seaweeds (Rhodophyceae). Through a purification process the product obtained has an ash content essentially lower than that of bacteriological agar. This process also has decreased the small oligosaccharides fraction and increased the agarose contents. The physicochemical, biochemical and bacteriological properties of this product are strictly controlled to guarantee its exceptional behavior in biochemical, bacteriological and mycological applications.

USES: in Biochemistry: Immunodiffusion: To be used as support
Electrophoresis: Excellent as support.
Cell Culture: Excellent for tissues and cell culture media.
Bacteriology: Essentially free of impurities that could interfer with other products in culture media formulation. Free of bacterial inhibitors. Recommended in media to be used for determination of the susceptibility of certain microorganisms or bacteriostatic drugs.
Mycology: For assimilation test in yeasts and molds. For susceptibility est for fungicidal and fungistatic drugs.

BIBLIOGRAPHY

* R.J. Wieme (1965). "Agar gel electrophoresis." Elsevier, Amsterdam.
* D. Freifelder. "Physical Biochemistry: Applications to Biochemistry and Molecular Biology." Freeman and Col. San Francisco.

SPECIFICATIONS:

PURIFIED AGAR SQA-20000

Electroendosmosis(EEO) (1)
<0.450

Moisture
<10.0%

Ash
<1.6%

Gel strength (1.5% - Nikkan)
700-1200 gr/cm2

Gelling Point (1.5% gel)
<32 - 37.5 °C

Melting point (1.5% gel)
<80 - 95 °C

Clarity 1.5% sol
<100 NP

pH (1.5%)
5.8-7.0

Colorimetry (absorbance) 430 nm
<0.150

(1) Wieme method pH 8.4

SEAQUALITE SQA-21000E AND SQA-21000A BACTERIOLOGICAL GRADE AGAR:

Bacteriological agar is a gelling agent used in the preparation of culture media and in other bacteriological applications.

The main advantage is the absence of inhibitors which could hinder optimal development of microorganisms. In addition, bacteriological agar also possesses other attributes such as transparency, high hysteresis and very reliable reproducibility.

Two types of bacteriological agar coexist in the market: European and American varieties. The first type has a higher gel strength and is used in concentrations of 1.0% to 1.2%, American-type agar gives lower gel strength and is utilized at concentrations ranging from 1.2% to 1.6%.

Each batch produced is thoroughly tested for biological performance against a battery of known bacterial cultures in order to ensure proper growth characteristics and the absence of inhibitors. Additional QC test are carried out to be certain that each batch meets established physical and chemical specifications.

BACTERIOLOGICAL GRADE AGAR

SPECIFICATIONS:

SeaQualite SQA-21000E

SeaQualite SQA-21000A

European Type

American Type

Moisture
<10.0%

<10.0%

Ash <4.5%
<6.5%

Gel strength (1.5% - Nikkan) 800-1100 gr/cm2
600-750 gr/cm2

Gelling Point (1.5% gel) 35°C ±3°C
35°C ±3°C

Melting point (1.5% gel) 85°C ±5°C
85°C ±5°C

Clarity 1.5% (HACH) < 12 NTU
< 12 NTU

pH (1.5%) before autoclaving 7.0 ±0.4
7.0 ±0.4

pH (1.5%) after autoclaving 6.5 ±0.4
6.5 ±0.4

Colorimeter (absorbance) 430 nm <0.200
<0.200

The particle size of both types is 95% pass mesh 60 ASTM

SQA-220000-1 Agar Technical Grade, Type B (40/60 Mesh) and SQA220000-2 Agar Technical Grade, Type B (80/100 Mesh)
General Description: Derived from Gelidium seaweeds
Primary Application: Use in microbiological and tissue culture media

Physical and Chemical Characteristics
Color: White to light cream
Particle Size: 40/60, 80/100 mesh sieve
Moisture: 20% or less
Gel Strength: 600-1100 g/cm2 (Nican, 1.5% sol
Melting Point: 82-88C (1.5% sol)
pH in Gel: 6.0- 8.0 ( 1.5% )
Turbidity: >50 NTU
Ash: 6.5% or less
Acid insoluble ash: 0.5% or less
Aresenic: less than 3 ppm
Lead: less than 5 ppm
Heavy metals: less than 40 ppm
Solubility: In boiling water

Microbiological Analysis:
Total plate count: <5000/g
Coliforms: < 3/g
E. Coli: Nil
Yeasts and molds: <100/g

Ordering/Price Information - Please see Page 57 or the index.

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PURIFIED AGAR SQA-20000
Electroendosmosis(EEO) (1)	<0.450
Moisture	<10.0%
Ash	<1.6%
Gel strength (1.5% - Nikkan)	700-1200 gr/cm2
Gelling Point (1.5% gel)	<32 - 37.5 °C
Melting point (1.5% gel)	<80 - 95 °C
Clarity 1.5% sol	<100 NP
pH (1.5%)	5.8-7.0
Colorimetry (absorbance) 430 nm	<0.150

	SeaQualite SQA-21000E	SeaQualite SQA-21000A
	European Type	American Type
Moisture	<10.0%	<10.0%
Ash	<4.5%	<6.5%
Gel strength (1.5% - Nikkan)	800-1100 gr/cm2	600-750 gr/cm2
Gelling Point (1.5% gel)	35°C ±3°C	35°C ±3°C
Melting point (1.5% gel)	85°C ±5°C	85°C ±5°C
Clarity 1.5% (HACH)	< 12 NTU	< 12 NTU
pH (1.5%) before autoclaving	7.0 ±0.4	7.0 ±0.4
pH (1.5%) after autoclaving	6.5 ±0.4	6.5 ±0.4
Colorimeter (absorbance) 430 nm	<0.200	<0.200